Information about Zika virus testing

This page contains information regarding Zika virus testing.

Page last updated: 18 December 2020

What types of tests are available to detect Zika virus?

There are currently two testing methods available for detection of Zika virus:

  1. Detection of Zika virus genetic material (PCR testing): This test looks for evidence of the genetic material (RNA) of Zika virus. The test is most sensitive in the first 7 days after symptoms start when performed on blood, and in the first 14 days when performed on urine. The major limitation of this test is that genetic material can be detected for only a short time after the start of the illness. PCR may be more difficult to interpret in people without symptoms.
  2. Detection of antibodies against Zika virus (serology): This blood test measures antibodies against the virus that are developed as part of the human body's reaction to infection. Antibodies begin to form approximately one week after the start of symptoms. This means waiting an appropriate time following potential exposure to Zika virus before ordering the test. This test is usually done on 2 blood samples, taken 2 weeks apart, to show a rise in the level of antibodies from one sample to the next. The major advantage of a serology test is that it remains positive for several months or longer after infection. The major limitations are that it can be slow to perform and can cross-react with other viruses related to Zika virus. A positive result may represent a previous exposure to another virus, such as dengue, or past vaccination (e.g. to yellow fever). This can make interpreting the result difficult. Additional testing can be performed in specialist laboratories to clarify the result.

Your usual pathology collection centre will collect the samples. They will then be sent to the most appropriate specialist testing centre.

Please give all relevant information to your health care provider. This will include mosquito bite exposure, past vaccinations or illnesses and recent (and past) travel history.

What do the Zika virus test results mean?

A positive PCR test for Zika virus genetic material means a current or very recent infection. Serology samples taken at least 2 weeks apart may be used to confirm this.

A negative PCR test for Zika virus genetic material may mean that there was no infection OR that the individual was infected but when the patient's sample was collected the virus was no longer present in the tested bodily fluid (blood or urine). Serology would then be used to decide if an infection was likely to have occurred.

A positive test for Zika virus by serology may be difficult to interpret or may require more extensive testing. Your health care provider in consultation with the pathologist will be able to assess if a Zika virus infection is likely.

A negative test for Zika virus by serology means either that the test was done too early in the disease course (no antibodies have yet been made and are not present in the collected sample), or that there was no infection. A negative serology test performed more than one to two months following return from travel indicates that there was no infection.

Who can get tested for Zika virus?

If you are worried about the risk of infection with Zika virus during travel to a Zika area of risk, or through sexual transmission please contact your health care provider.

Generally only people with symptoms of Zika virus infection and who have a compatible exposure history need to be tested.

Zika virus infections in pregnant women may lead to serious outcomes for babies. Testing can be offered to pregnant women who may have been exposed to Zika through travel or sexual transmission.

A positive test for Zika virus in a pregnant woman does not mean that her baby is infected or harmed; however she will need further specialist assessment.

In some circumstances testing will be offered if a couple is planning pregnancy following travel to a Zika virus affected country or a potentially affected country, including if there are concerns about the consequences of delaying pregnancy for the recommended time periods.

Further information on testing is available for health professionals. Please refer to: